3L4E

1.5A Crystal Structure of a Putative Peptidase E Protein from Listeria monocytogenes EGD-e

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-12
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 65 2 2
Unit cell lengths	67.821, 67.821, 155.169
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.415 - 1.500
R-factor	0.1692
Rwork	0.169
R-free	0.22220
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.983
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.4_115))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.050	0.646
Number of reflections	34751
<I/σ(I)>	36.4	2.57
Completeness [%]	99.9	99.7
Redundancy	9.9	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	2.5M NH4 Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	4	298	2.5M NH4 Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K