3L49
CRYSTAL STRUCTURE OF ABC SUGAR TRANSPORTER SUBUNIT FROM Rhodobacter sphaeroides 2.4.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-12 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.310, 75.712, 77.438 |
| Unit cell angles | 106.41, 115.49, 96.71 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.19504 |
| Rwork | 0.193 |
| R-free | 0.25436 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.234 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.140 | |
| Number of reflections | 56552 | |
| <I/σ(I)> | 3.5 | 1.1 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | 200MM SODIUM ACETATE, PH 4.6, 100MM TRIS-HCL, PH 8.5, 10% GLYCEROL, 30% PEG4000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
