3L47
Crystal Structure of the Anopheles gambiae Odorant-binding Protein 22a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-18 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.871, 37.920, 43.889 |
| Unit cell angles | 90.00, 98.92, 90.00 |
Refinement procedure
| Resolution | 29.828 - 2.506 |
| R-factor | 0.185 |
| Rwork | 0.180 |
| R-free | 0.22600 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.156 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE (2.13) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.760 | 2.500 |
| Rmerge | 0.064 | 0.052 | 0.140 |
| Number of reflections | 4107 | ||
| <I/σ(I)> | 32.2 | ||
| Completeness [%] | 99.8 | 100 | 99.5 |
| Redundancy | 6.2 | 5.7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Ammonium citrate tribasic, MPD, pH 7.0, vapor diffusion, hanging drop, temperature 298K |
