3L3H

X-ray crystal structure of the F6A mutant of influenza A acid polymerase epitope PA224 bound to murine H2-Db MHC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-04-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.51478
Spacegroup name	P 43 21 2
Unit cell lengths	56.068, 56.068, 275.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 2.700
R-factor	0.22673
Rwork	0.220
R-free	0.28409
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cc5
RMSD bond length	0.006
RMSD bond angle	0.888
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.091	0.317
Number of reflections	12799
<I/σ(I)>	18.4	5.1
Completeness [%]	98.2	98
Redundancy	4.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1M citrate, 0.2M ammonium acetate, 25% PEG4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K