3L2M
X-ray Crystallographic Analysis of Pig Pancreatic Alpha-Amylase with Alpha-cyclodextrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-06-17 |
| Detector | SDMS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.650, 114.880, 118.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.300 - 1.970 |
| R-factor | 0.13005 |
| Rwork | 0.127 |
| R-free | 0.16021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.387 |
| Data reduction software | SDMS (Detector System) |
| Data scaling software | SDMS (Detector System) |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 2.070 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.068 | 0.178 |
| Number of reflections | 62592 | |
| <I/σ(I)> | 19.83 | 3.07 |
| Completeness [%] | 84.6 | 36.6 |
| Redundancy | 4.84 | 1.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.75 | 298 | 0.010 M cacodylate, 0.002 M calcium chloride, soaking of alpha-cyclodextrin, pH 6.75, EVAPORATION, temperature 298K |
