3L2L
X-ray Crystallographic Analysis of Pig Pancreatic Alpha-Amylase with Limit Dextrin and Oligosaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-09-02 |
| Detector | SDMS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.590, 114.820, 118.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.010 - 2.110 |
| R-factor | 0.11929 |
| Rwork | 0.115 |
| R-free | 0.15720 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.487 |
| Data reduction software | SDMS (Detector System) |
| Data scaling software | SDMS (Detector System) |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.010 | 2.220 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.052 | |
| Number of reflections | 47147 | |
| <I/σ(I)> | 10.93 | 3.91 |
| Completeness [%] | 82.6 | 28.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.75 | 298 | 0.010 M cacodylate, 0.002 M calcium chloride, pH 6.75, EVAPORATION, temperature 298K |
