3L1X

Crystal Structure of U-box Domain of Human E4B Ubiquitin Ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97918
Spacegroup name	P 4 3 2
Unit cell lengths	83.395, 83.395, 83.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.070 - 2.600
R-factor	0.1913
Rwork	0.185
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2EZJ
RMSD bond length	0.002
RMSD bond angle	0.588
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.074	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.139	0.655
Number of reflections	3328
<I/σ(I)>	46.18	7.29
Completeness [%]	99.9	99
Redundancy	37	36.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	2M TACSIMATE, VAPOR DIFFUSION, HANGING DROP, temperature 295K