3L1W
The crystal structure of a functionally unknown conserved protein from Enterococcus faecalis V583
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.565, 68.558, 104.245 |
| Unit cell angles | 89.88, 89.79, 60.07 |
Refinement procedure
| Resolution | 32.610 - 1.600 |
| R-factor | 0.1681 |
| Rwork | 0.167 |
| R-free | 0.19750 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.057 | 0.729 |
| Number of reflections | 210181 | |
| <I/σ(I)> | 21.6 | 1.3 |
| Completeness [%] | 97.0 | 94.9 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 297 | 1.0M (NH4)2SO4, 0.1M Bis-Tris, 1%w/v PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
