3L1R
X-ray structure of the mutant lys300met of polyamine oxidase from zea mays in complex with spermidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Detector technology | CCD |
| Collection date | 2009-07-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 137.676, 137.676, 189.148 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.500 - 3.200 |
| R-factor | 0.19367 |
| Rwork | 0.192 |
| R-free | 0.21627 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kpf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.238 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0043) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.195 | 0.578 |
| Number of reflections | 34784 | |
| <I/σ(I)> | 8.6 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 50% SATURATED AMMONIUM SULFATE SOLUTION, 0.1M SODIUM ACETATE PH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
