3L1G
Human AlphaB crystallin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.49 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 50.950, 50.950, 104.430 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.878 - 3.320 |
R-factor | 0.301 |
Rwork | 0.299 |
R-free | 0.32700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.218 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.4_4) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 18.880 | 3.230 | |
High resolution limit [Å] | 3.150 | 14.090 | 3.150 |
Rmerge | 0.055 | 0.038 | 0.529 |
Number of reflections | 2777 | 20 | 207 |
<I/σ(I)> | 19.05 | 35.1 | 3.2 |
Completeness [%] | 98.3 | 51.3 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.08M SODIUM ACETATE pH 4.6, 20% GLYCEROL, 1.8M AMMONIUM SULFATE, vapor diffusion, sitting drop, temperature 298K |