3L15
Human Tead2 transcriptional factor
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-19 |
Detector | SBC-3 |
Wavelength(s) | 0.9798 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.144, 61.567, 80.472 |
Unit cell angles | 90.00, 117.27, 90.00 |
Refinement procedure
Resolution | 33.738 - 2.000 |
R-factor | 0.1918 |
Rwork | 0.189 |
R-free | 0.24450 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.056 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.100 | 0.064 | 0.711 |
Number of reflections | 34385 | ||
<I/σ(I)> | 10.5 | ||
Completeness [%] | 96.1 | 97.8 | 70.9 |
Redundancy | 4.9 | 4.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 0.1 M Tris, 2.5 M Sodium Formate, 100 mM NaCl, 2 mM MgCl2, 1 mM TCEP, 5% (w/v) Glycerol;, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |