3L0R
Crystal Structure of Salivary Cystatin from the Soft Tick Ornithodoros moubata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.805, 69.805, 133.994 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.950 - 2.450 |
| R-factor | 0.18937 |
| Rwork | 0.188 |
| R-free | 0.22312 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lh4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.256 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.334 | |
| Number of reflections | 14369 | |
| <I/σ(I)> | 27.3 | 2.4 |
| Completeness [%] | 98.8 | 90 |
| Redundancy | 5.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES pH 7.5, 1.5M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
