3L0A

Crystal structure of Putative exonuclease (RER070207002219) from Eubacterium rectale at 2.19 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-14
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97936,0.97922
Spacegroup name	P 63
Unit cell lengths	88.248, 88.248, 69.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.108 - 2.190
R-factor	0.21
Rwork	0.208
R-free	0.25000
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.561
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.108	44.108	2.270
High resolution limit [Å]	2.190	4.700	2.190
Rmerge	0.067	0.047	0.576
Number of reflections	15931	1658	1608
<I/σ(I)>	17.84	45.8	2
Completeness [%]	100.0	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.2000M MgCl2, 30.0000% PEG-4000, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K