3L0A
Crystal structure of Putative exonuclease (RER070207002219) from Eubacterium rectale at 2.19 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-14 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91162,0.97936,0.97922 |
| Spacegroup name | P 63 |
| Unit cell lengths | 88.248, 88.248, 69.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.108 - 2.190 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.561 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.108 | 44.108 | 2.270 |
| High resolution limit [Å] | 2.190 | 4.700 | 2.190 |
| Rmerge | 0.067 | 0.047 | 0.576 |
| Number of reflections | 15931 | 1658 | 1608 |
| <I/σ(I)> | 17.84 | 45.8 | 2 |
| Completeness [%] | 100.0 | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2000M MgCl2, 30.0000% PEG-4000, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
