3L01
Crystal structure of monomeric glycogen synthase from Pyrococcus abyssi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-07 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.98020 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.604, 119.017, 141.101 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.910 - 2.600 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bis |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.570 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.056 | 0.450 |
| Number of reflections | 44800 | |
| <I/σ(I)> | 10 | 2.8 |
| Completeness [%] | 85.0 | 83 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | ammonium sulfate, potassium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
