3KZF
Structure of Giardia Carbamate Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.770, 85.410, 102.100 |
| Unit cell angles | 90.00, 106.80, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| Rwork | 0.227 |
| R-free | 0.28305 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e19 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.100 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.095 | 0.193 |
| Number of reflections | 20419 | |
| Completeness [%] | 88.4 | 87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350 and 0.2 M ammonium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0 K |
