3KZD
Crystal Structure of Free T-cell Lymphoma Invasion and Metastasis-1 PDZ Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 44.782, 44.782, 70.799 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.010 - 1.300 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.995 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.9L) |
| Phasing software | PHASER (1.3.3) |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.025 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.050 | 0.336 |
| Total number of observations | 19857 | |
| Number of reflections | 20526 | |
| <I/σ(I)> | 18.5 | 5.4 |
| Completeness [%] | 98.5 | 96.5 |
| Redundancy | 10.25 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 288 | 20% (w/v) PEG 3350, 0.2 M NaSCN, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
