3KZC

Crystal structure of N-acetyl-L-ornithine transcarbamylase

Replaces:  1YH0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	95
Detector technology	CCD detector
Collection date	2004-12-04
Detector	Dual Quantum 4
Wavelength(s)	0.9186
Spacegroup name	I 21 3
Unit cell lengths	128.039, 128.039, 128.039
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.270 - 2.200
Rwork	0.218
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1akm
RMSD bond length	0.006
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.066	0.460
Number of reflections	17844
<I/σ(I)>	28.5	4.8
Completeness [%]	99.7	100
Redundancy	9.8	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6	291	Lithium sulfate, Bis-tris, PEG3350, pH 6.0, hanging drop, temperature 291K