3KZ7
C-terminal domain of Murine FKBP25 rapamycin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.649, 85.649, 53.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.303 - 1.950 |
| R-factor | 0.1773 |
| Rwork | 0.175 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pbk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.075 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.000 | 1.880 |
| High resolution limit [Å] | 1.680 | 1.880 |
| Rmerge | 0.068 | 0.120 |
| Number of reflections | 23011 | |
| <I/σ(I)> | 6.5 | 12.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 2.4M ammonium sulfate, 120mM Na citrate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
