3KZ5
Structure of cdomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Detector technology | CCD |
| Collection date | 2009-11-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.010, 45.400, 36.330 |
| Unit cell angles | 90.00, 100.44, 90.00 |
Refinement procedure
| Resolution | 43.770 - 1.580 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.23400 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.850 |
| High resolution limit [Å] | 1.580 |
| Rmerge | 0.050 |
| Number of reflections | 18124 |
| <I/σ(I)> | 10.1 |
| Completeness [%] | 91.8 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG 4000, sodium acetate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
