3KYQ
Lipid-induced Conformational Switch Controls Fusion Activity of Longin Domain SNARE Ykt6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.810, 59.710, 108.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.443 |
| R-factor | 0.20516 |
| Rwork | 0.199 |
| R-free | 0.26137 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bw6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.060 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.506 |
| High resolution limit [Å] | 2.443 | 2.443 |
| Rmerge | 0.067 | 0.336 |
| Number of reflections | 6788 | |
| <I/σ(I)> | 10 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 2M (NH4)2SO4, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
