3KYO

Crystal structure of HLA-G presenting KLPAQFYIL peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-12
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	58.606, 85.982, 111.570
Unit cell angles	90.00, 95.61, 90.00

Refinement procedure

Resolution	43.000 - 1.700
R-factor	0.18382
Rwork	0.182
R-free	0.22645
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ydp
RMSD bond length	0.022
RMSD bond angle	1.888
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.082	0.589
Number of reflections	119833
<I/σ(I)>	33.4	2.9
Redundancy		3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277	16% PEG 3350, 0.2M potassium formate, 0.1M HEPES, 10mM cobalt chloride, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K