3KYN

Crystal structure of HLA-G presenting KGPPAALTL peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-03
Detector	MAR CCD 165 mm
Spacegroup name	P 32 2 1
Unit cell lengths	76.874, 76.874, 151.851
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.270 - 2.400
R-factor	0.22089
Rwork	0.217
R-free	0.29913
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ydp
RMSD bond length	0.011
RMSD bond angle	1.406
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.300	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.061	0.416
Number of reflections	20378
<I/σ(I)>	24.4	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277	18% PEG 3350, 0.2M potassium formate, 0.1M HEPES, 10mM cobalt chloride, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K