3KYL
Structure of the catalytic subunit of telomerase bound to its RNA template and telomeric DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 175 |
| Collection date | 2009-04-15 |
| Wavelength(s) | 0.97880 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.231, 52.782, 101.609 |
| Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
| Resolution | 25.510 - 2.700 |
| R-factor | 0.24456 |
| Rwork | 0.242 |
| R-free | 0.28711 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.833 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.510 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.114 | 0.458 |
| Number of reflections | 19815 | |
| <I/σ(I)> | 7.9 | 2.3 |
| Completeness [%] | 93.3 | 92.3 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M Hepes 7.5, 10% PEG 4K, 0.2M KCl, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
