3KYJ
Crystal structure of the P1 domain of CheA3 in complex with CheY6 from R. sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.718, 62.011, 48.911 |
| Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
| Resolution | 37.942 - 1.400 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20700 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.4_129) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 2.590 | 1.200 |
| Rmerge | 0.053 | 0.048 | 0.483 |
| Number of reflections | 68797 | ||
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 85.8 | 100 | 36.2 |
| Redundancy | 5.3 | 6.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 295 | 100mM Bicine, pH 9, 1M LiCl, 20% (w/v) PEG 6000, vapor diffusion, temperature 295K |
