3KXS
Crystal structure of HBV capsid mutant dimer (oxy form), strain adyw
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 |
| Unit cell lengths | 104.162, 104.162, 86.917 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.320 - 2.250 |
| R-factor | 0.21966 |
| Rwork | 0.217 |
| R-free | 0.26418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgt |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.926 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.076 | 0.572 |
| Number of reflections | 228368 | |
| <I/σ(I)> | 41.8 | 5.34 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG1500, Calcium acetate, sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
