3KXM
Crystal structure of Z. mays CK2 kinase alpha subunit in complex with the inhibitor K74
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976250 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.185, 60.533, 45.873 |
| Unit cell angles | 90.00, 103.04, 90.00 |
Refinement procedure
| Resolution | 69.338 - 1.750 |
| R-factor | 0.227 |
| Rwork | 0.224 |
| R-free | 0.28200 |
| Structure solution method | Rigid body in an isomorphous cell |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.990 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.340 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.065 | 0.379 |
| Total number of observations | 13330 | |
| Number of reflections | 35009 | |
| <I/σ(I)> | 17.2 | 1.9 |
| Completeness [%] | 91.2 | 62.6 |
| Redundancy | 5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG 4000, 0.2M Na-acetate, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
