3KXE

A conserved mode of protein recognition and binding in a ParD-ParE toxin-antitoxin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-09
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	65.735, 72.707, 76.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.960 - 2.600
R-factor	0.2393
Rwork	0.237
R-free	0.29160
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.540
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.960	2.660
High resolution limit [Å]	2.600	2.600
Number of reflections	11711
Completeness [%]	99.8	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM MES, 100 mM (NH4)2HPO3, 10% PEG 20,000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM MES, 100 mM (NH4)2HPO3, 10% PEG 20,000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K