3KWR

Crystal structure of Putative RNA-binding protein (NP_785364.1) from LACTOBACILLUS PLANTARUM at 1.45 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-30
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 62
Unit cell lengths	104.869, 104.869, 35.979
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.670 - 1.450
R-factor	0.164
Rwork	0.163
R-free	0.18100
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.485
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.670		1.500
High resolution limit [Å]	1.450	3.120	1.450
Rmerge	0.041	0.025	0.592
Number of reflections	40229	7844	6918
<I/σ(I)>	16.58	53.8	1.6
Completeness [%]	97.0	99.1	90.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	0.8000M (NH4)2SO4, 0.1M Bicine pH 9.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K