3KWO
Crystal Structure of Putative Bacterioferritin from Campylobacter jejuni
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | H 3 |
| Unit cell lengths | 91.715, 91.715, 202.288 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.780 - 1.985 |
| R-factor | 0.1462 |
| Rwork | 0.144 |
| R-free | 0.19650 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.375 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.780 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.135 | |
| Number of reflections | 43678 | |
| <I/σ(I)> | 9.54 | 2.51 |
| Completeness [%] | 98.5 | 76.3 |
| Redundancy | 6.3 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2 M Zinc acetate, 0.1 M Imidazole pH 8.0, 20% (v/v) 1,4-bdiolutaiediol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
