3KWI
X-ray structure of NS1 effector domain W187Y mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.991, 60.236, 132.655 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.120 - 2.210 |
| R-factor | 0.22362 |
| Rwork | 0.222 |
| R-free | 0.25762 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ee9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.003 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.048 | 0.175 |
| Number of reflections | 18310 | |
| <I/σ(I)> | 51.4 | 62.2 |
| Completeness [%] | 99.0 | 90.5 |
| Redundancy | 10 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 277 | 0.3M magnesium formate and 0.1M Bis-Tris , pH 5.5, EVAPORATION, temperature 277K |
