3KVT
TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 67.191, 67.191, 138.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1AP61 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 * | 0.401 * |
| Total number of observations | 126110 * | |
| Number of reflections | 11135 | |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 2.5 (M) | |
| 2 | 1 | reservoir | bis-Tris-propane | 0.1 (M) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | 0.15 (M) | ||
| 5 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) | |
| 6 | 1 | drop | isopropanol | 24 (%) | |
| 7 | 1 | drop | 0.2 (M) | ||
| 8 | 1 | drop | HEPES | 0.1 (M) | pH7.5 |
| 9 | 1 | drop | dithiothreitol | 1 (mM) |
