3KVS
The high resolution structure of C-Phycocyanin from Galdieria Sulphuraria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0053 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 105.300, 105.300, 174.021 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.986 - 1.500 |
| R-factor | 0.1328 |
| Rwork | 0.131 |
| R-free | 0.17530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3brp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.149 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.990 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.069 | 0.295 |
| Number of reflections | 51816 | |
| <I/σ(I)> | 13.3 | 4.3 |
| Completeness [%] | 87.9 | 89.9 |
| Redundancy | 5.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 9.5% PEG 2000,0.1M MES, 0.1M MGCL2, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
