3KVP

Crystal Structure of Uncharacterized protein ymzC Precursor from Bacillus subtilis, Northeast Structural Genomics Consortium Target SR378A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-07
Detector	ADSC QUANTUM 315
Spacegroup name	P 32 2 1
Unit cell lengths	66.390, 66.390, 175.573
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.968 - 2.404
R-factor	0.1959
Rwork	0.194
R-free	0.23720
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.150
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.400
Rmerge	0.097
Number of reflections	47159
<I/σ(I)>	22.4
Completeness [%]	99.3
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: Zn-acetate 0.2M, PEG3350 20%, ammonium sulfate 1.0M., VAPOR DIFFUSION, HANGING DROP, temperature 277K