3KUL
Kinase domain of human ephrin type-A receptor 8 (EPHA8)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.140, 41.879, 145.815 |
| Unit cell angles | 90.00, 92.46, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.18985 |
| Rwork | 0.187 |
| R-free | 0.24389 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rei |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.325 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 29557 | |
| <I/σ(I)> | 20.6 | 3.15 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | WELL SOLUTION: 20% PEG 8000, 0.2 M NH4 SULPHATE, 0.1 M HEPES PH 7.5. PROTEIN SOLUTION: 20 MG/ML, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
