3KUD
Complex of Ras-GDP with RafRBD(A85K)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97935 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 91.130, 91.130, 277.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.680 - 2.150 |
| R-factor | 0.23809 |
| Rwork | 0.237 |
| R-free | 0.26381 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gua |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.571 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.089 | 0.669 |
| Number of reflections | 24583 | |
| <I/σ(I)> | 14.76 | 1.83 |
| Completeness [%] | 92.8 | 82.2 |
| Redundancy | 3.83 | 1.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1.3M Na-Malonat pH 6.0, 100 mM MES pH 6.1, 4% Betaine, 2% Sarcosine, 2% N,N-dimethylglycine, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
