3KU5
Crystal structure of a H2N2 influenza virus hemagglutinin, human like
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03333 |
| Spacegroup name | P 63 |
| Unit cell lengths | 70.694, 70.694, 236.751 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.730 |
| R-factor | 0.18927 |
| Rwork | 0.187 |
| R-free | 0.22251 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.437 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.086 | 0.534 |
| Number of reflections | 67767 | |
| <I/σ(I)> | 13 | 3.1 |
| Completeness [%] | 97.7 | 99 |
| Redundancy | 6.5 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 295 | 26% PEG 3000, 0.1M Tris, pH 7.8, vapor diffusion, sitting drop, temperature 295K |
