3KTZ
Structure of GAP31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2003-10-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.373, 44.395, 137.270 |
| Unit cell angles | 90.00, 98.38, 90.00 |
Refinement procedure
| Resolution | 47.350 - 1.600 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.350 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.036 | |
| Number of reflections | 228644 | |
| Completeness [%] | 99.2 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 300 | 100 mM Tris-HCl, pH 8.5, 2.0 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 300 | 100 mM Tris-HCl, pH 8.5, 2.0 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 300 | 100 mM Tris-HCl, pH 8.5, 2.0 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
