3KTV
Crystal structure of the human SRP19/S-domain SRP RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 100.120, 100.120, 293.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.010 - 3.800 |
| R-factor | 0.293 |
| Rwork | 0.291 |
| R-free | 0.32900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lng |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.352 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.200 | 4.010 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 14852 | |
| <I/σ(I)> | 7.3 | 1.3 |
| Completeness [%] | 96.1 | 97.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 100 mM NaOAc, 0.75 M KF, 2.2 M (NH4)2SO4, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaOAc | ||
| 2 | 1 | 1 | KF | ||
| 3 | 1 | 1 | (NH4)2SO4 | ||
| 4 | 1 | 2 | NaOAc | ||
| 5 | 1 | 2 | KF | ||
| 6 | 1 | 2 | (NH4)2SO4 |
