3KTA
Structural Basis for Adenylate Kinase Activity in ABC ATPases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.000, 86.100, 70.100 |
| Unit cell angles | 90.00, 116.60, 90.00 |
Refinement procedure
| Resolution | 35.435 - 1.627 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.20620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.138 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.645 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 84052 | |
| Completeness [%] | 95.7 | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 298 | BisTRIS, 16% PEG3350, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
