3KSX
The alkanesulfonate-binding protein SsuA from Xanthomonas axonopodis pv. citri bound to MOPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2009-10-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.46 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.042, 86.446, 47.015 |
| Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
| Resolution | 26.140 - 1.700 |
| R-factor | 0.16579 |
| Rwork | 0.163 |
| R-free | 0.21303 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e4r |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.140 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.163 | |
| Number of reflections | 26985 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 95.4 | 96.6 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.2 M MOPS pH 6.8, 0.1 M NaCl, 1.6 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
