3KSS
Structure and Mechanism of the Heavy Metal Transporter CusA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.03332 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 180.363, 180.363, 287.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.284 - 3.880 |
| R-factor | 0.263 |
| Rwork | 0.260 |
| R-free | 0.32500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.995 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.030 |
| High resolution limit [Å] | 3.880 | 3.880 |
| Rmerge | 0.086 | 0.424 |
| Number of reflections | 15855 | |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 98.8 | 99.6 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 8% PEG4000, 0.1M MES(6.5), 0.4M (NH4)2SO4 , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
