3KSO
Structure and Mechanism of the Heavy Metal Transporter CusA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.377 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 179.986, 179.986, 286.018 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.469 - 4.367 |
| R-factor | 0.271 |
| Rwork | 0.268 |
| R-free | 0.32100 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.252 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.510 |
| High resolution limit [Å] | 4.350 | 4.350 |
| Rmerge | 0.059 | 0.449 |
| Number of reflections | 15666 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 95.1 | 94.1 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 8% PEG4000, 0.1M MES(6.5), 0.4M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
