3KS2

Crystal Structure of Type-III Secretion Chaperone IpgC from Shigella flexneri (residues 10-155)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	140.496, 71.474, 171.012
Unit cell angles	90.00, 93.86, 90.00

Refinement procedure

Resolution	46.726 - 3.300
R-factor	0.2605
Rwork	0.259
R-free	0.29620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gyz
RMSD bond length	0.011
RMSD bond angle	1.285
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.300
Number of reflections	45608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.2M magnesium chloride hexahydrate, 0.1M HEPES, 25% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K