3KPX

Crystal Structure Analysis of photoprotein clytin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	43.392, 68.932, 115.348
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.306 - 1.899
R-factor	0.1731
Rwork	0.171
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f8p
RMSD bond length	0.011
RMSD bond angle	1.186
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.899
Number of reflections	14018
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP