3KPC
Crystal Structure of the CBS domain pair of protein MJ0100 in complex with 5 -methylthioadenosine and S-adenosyl-L-methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Collection date | 2009-09-19 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 50.964, 71.483, 86.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.390 - 1.790 |
| R-factor | 0.217 |
| Rwork | 0.216 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.159 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 86.390 |
| High resolution limit [Å] | 1.790 |
| Number of reflections | 14353 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 291 | 10% PEG 6000, 0.1 M TRIS pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
