3KOP
Crystal structure of Protein with a cyclophilin-like fold (YP_831253.1) from Arthrobacter sp. FB24 at 1.90 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97963 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.294, 98.102, 84.503 |
| Unit cell angles | 90.00, 93.19, 90.00 |
Refinement procedure
| Resolution | 29.907 - 1.900 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.19600 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.401 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.907 | 29.910 | 1.950 |
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.086 | 0.041 | 0.498 |
| Total number of observations | 2479 | 16367 | |
| Number of reflections | 88263 | ||
| <I/σ(I)> | 9 | 12.3 | 2 |
| Completeness [%] | 99.5 | 94.4 | 100 |
| Redundancy | 2.6 | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 10.0000% MPD, 0.1M Acetate pH 5.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
