3KNU

Crystal structure of tRNA (guanine-N1)-methyltransferase from Anaplasma phagocytophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9774
Spacegroup name	P 21 21 2
Unit cell lengths	104.291, 104.304, 99.105
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.250
R-factor	0.205
Rwork	0.203
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ief
RMSD bond length	0.014
RMSD bond angle	1.295
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.064	0.584
Number of reflections	52092
<I/σ(I)>	30.95	3.4
Completeness [%]	100.0	99.9
Redundancy	7.2	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	289	21.0 mg/mL protein, expression tag not cleaved prior to crystallization; 0.1 M Na citrate pH 4.0, 8% PEG 3350, 10% glycerol, crystal tracking ID 205756f1 and q1x5b1-7, VAPOR DIFFUSION, SITTING DROP, temperature 289K