3KNU
Crystal structure of tRNA (guanine-N1)-methyltransferase from Anaplasma phagocytophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 104.291, 104.304, 99.105 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ief |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.295 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.064 | 0.584 |
| Number of reflections | 52092 | |
| <I/σ(I)> | 30.95 | 3.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.2 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 289 | 21.0 mg/mL protein, expression tag not cleaved prior to crystallization; 0.1 M Na citrate pH 4.0, 8% PEG 3350, 10% glycerol, crystal tracking ID 205756f1 and q1x5b1-7, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
