Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99184, 0.91740, 0.91706 |
| Spacegroup name | P 31 |
| Unit cell lengths | 60.910, 60.910, 42.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.530 - 1.400 |
| R-factor | 0.1653 |
| Rwork | 0.164 |
| R-free | 0.19490 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.086 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.530 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 34179 | |
| Completeness [%] | 99.5 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | ammonium sulfate, imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
