3KNA
M296I mutant of foot-and-mouth disease virus RNA-polymerase in complex with a template- primer RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.565, 93.565, 120.931 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.000 - 2.800 |
| R-factor | 0.24062 |
| Rwork | 0.239 |
| R-free | 0.27978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wne |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.430 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.600 |
| Number of reflections | 13868 | |
| <I/σ(I)> | 11.4 | 2.3 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 30% PEG 4000, 0.1M HEPES pH 7.0, 4% butyrolactone, 0.2M Magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
