3KN8
Crystal Structure of Haemophilus influenzae Y196A mutant Holo Ferric ion-Binding Protein A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-06 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.025, 75.573, 33.103 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.000 - 1.890 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d9v |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.155 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.000 | 2.030 |
| High resolution limit [Å] | 1.890 | 1.900 |
| Number of reflections | 20126 | |
| <I/σ(I)> | 9.94 | 2.02 |
| Completeness [%] | 92.3 | 92.32 |
| Redundancy | 3.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 28% PEG 550 MME, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
